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KMID : 0385020000160020149
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Korean Journal of Laboratory Animal Science
2000 Volume.16 No. 2 p.149 ~ p.157
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Germ-line Transmission of a Targeted Duplication Made in a Renin Gene by Gap-repair in Embryonic Stem Cells
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Abstract
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We have duplicated the two highly homologous mouse renin genes Ren-1d and Ren-2 of mouse embryonic stem (ES) cell line by using an OSDUPDEL targeting vector. A targeting construct for duplication was made using the same two flanking region of the Ren-2 and Ren-ld gene. The targeting frequency of duplication event was 1:200. Embryonic stem cells (derived 129/Ola mice) containing a duplicated renin gene that had been corrected in vitro in a planned manner by gap-repair were injected blastocysts obtained from C57BL/6ByJKist mice. The injected blastocytes were introduced into pseudopregnant female mice to complete their development. Eleven surviving pups were obtained. Three were chimeras: one male and two females. The male transmitted the embryonic stem cell genome containing the alteration in the renin gene to their offspring at high frequencies. These experiments demonstrate that a preplanned alteration in a chosen gene can be made in the gap-repair in an embryonic stem cell.
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